B.3.2. TIMELINE

B.3.2.
Transparent pot with a draining system

⌕ Can mycelium form on fermented HOW substrates that contain meat and other animal waste?

Run through: 12.08 – present;
Total batch weight: 2.25kg;
Bokashi fermentation proportions: approximately 1:10 ⌕ 250g Bokashi + 2kg HOW (of which 10% is chicken meat);
HOW was minced in a blender; anaerobic fermentation.

Experiment 28. 08. 2019: a repetition experiment


⌕ Will repeating the same experiment lead the same results?

⌕ Does HOW material structure change when HOW and the mycelium spawn are homogeneously mixed or layered?

In the weeks leading up to this experiment, HOW samples have not been developing very well. This experiment focuses on verifying if repeating the conditions of successful samples will give the same results. Additionally, mycelium forming direction and structure are tested.

Day 01 into the experiment. All samples. Left column: homogeneously mixed. Right column: layered samples. In all the pictures, the samples are arranged in the same way.
Day 05 into the experiment.
Day 13 into the experiment.
Day 15 into the experiment. Samples ready to be dried. Samples B.3.2.1A (mixed), B.3.1.1B & B.3.2.1B (layered) failed.
Day 15 into the experiment. Example of one of the samples.
8 medium Petri dishes were prepared, using HOW from all dried batches laying around at this point, in pairs. All 'A' samples were mixed, meaning that the ganoderma mycelium spawn is mixed homogeneously with the dried HOW. All 'B' samples were layered, which means that the mycelium spawn is laid at the bottom of the dish separate from the HOW substrate:



The batch samples from B.0.1 and B.1.1 grew the same as before. B.3.1 and B.3.2 were newly introduced and behaved differently in the repetition conditions. This could have to do with the composition of the dried HOW.

Mixed samples became slightly curved towards the top, while the layered samples formed flat. A cause of this could have been that mycelium always forms its net upwards, thereby constructing a denser mass and tension in the upper part of the dish.

Experiment 16. 09. 2019: colouring attempt 03


⌕ Can the HOW material be coloured?

⌕ Which pigments can be used without being decomposed by the fungi enzymes during mycelium forming?

This experiment focuses on testing if different colour dyes preserve during the mycelium forming stage, and if colour dye hinders mycelium forming when the HOW substrate is present.

15 medium Petri dishes were prepared; 10 dishes containing HOW substrates from all batches laying around at the time, and 5 control dishes. The red and blue silk dyes were poured on top of the dishes after the mycelium spawn and the HOW were mixed. As yellow dye, turmeric powder was mixed homogeneously in the mycelium control dishes.



Day 01. Red control & all samples
Day 01. Blue & yellow controls
Day 04. Blue & yellow controls
Day 09. Red control & all samples
Day 16 of the experiment. Examples (left to right): samples B.3.1.2B, B.3.2.2A, B.3.3.1B & Yellow B
The red silk dye preserved during mycelium forming in most situations.
The blue silk dye was decomposed during mycelium forming, the colour dissapeared until the end of the process.
Of the 3 yellow dye control dishes, the C sample didn't grow.

This experiment concluded that colouring the HOW material is possible but the exact conditions in which this must happen are yet to be researched.
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